DTI FabSpin Tissue DNA Extraction Kit

The DTI FabSpin Tissue DNA Extraction Kit is designed for DNA extraction and purification from a variety of from a variety of sample sources including blood, gram-negative bacteria, mammalian cul¬tured cells, plant tissue and animal tissue. This kit employs a proprietary lysis buffer in com¬bination with Spin Column membrane to efficiently purify genomic DNA from the biological sample. The protocol provides a simple method to achieve the rapid isolation of highly puri¬fied genomic DNA and the entire procedure can be accomplished within 20 minutes after tissue cell lysed. Using the kit about 10 μg of highly purified genomic DNA can be extracted from 50 – 200 μl of mammalian whole blood (with anticoagulant), 1 – 10 μl of nucleated erythrocyte in whole blood (with anticoagulant), 1.0 – 5.0 x 109 gram-negative bacteria, 1.0 x 105 – 1.0 x 107 cultured cells, 2 – 30 mg of mammalian tissues and 25 – 100 mg plant tissues. Genomic DNA prepared by this kit is suitable for a variety of applications, such as PCR, South¬ern blotting, RAPD, AFLP, RFLP and other molecular biology experiments.