RNAiso Plus

RNAiso Plus is a reagent designed for the quick and efficient isolation of total RNA from plant and animal tissues or cultured cells. The process involves adding RNAiso Plus to disrupt the tissues or cells, followed by adding chloroform, mixing, and centrifugation. This separates the solution into three layers: RNA in the upper transparent water layer, DNA in the semi-solid middle layer, and proteins, polysaccharides, and cell debris in the red organic solvent layer at the bottom. The RNA is collected from the upper layer and recovered through isopropanol precipitation. The entire RNA extraction process takes about 1 hour, and the extracted RNA is suitable for RT-PCR, Northern blotting, mRNA separation, and in vitro translation. The red color of the organic solvent layer makes it easy to distinguish the layers during the process. For RT-PCR, it is recommended to treat RNA samples with Recombinant DNase I (RNase-free) to eliminate any gDNA contamination.