Agarose electrophoresis is the most effective way to identify and separate DNA fragments. The purity of agarose directly affects the resolution of DNA and the clarity of electrophoresis results. If the agarose contains sugars, salts, or proteins, it will affect the migration speed of DNA in the gel and subsequently the reaction performance of recovered DNA fragments during enzymatic reactions. Hence, using high-quality agarose is important for the success of the experiment. DTI Agarose premium is a high-quality agarose product with high gel strength, suitable for making as low as 0.7% to as high concentration 3% agarose gel. When stained with EtBr, the electrophoretic separation performance is strong, bands are clear with minimal background, and it is suitable for electrophoresis of various DNA fragments. It is an economical and ideal grade for conventional agarose electrophoresis.
Gel Strength (1.0% gel)
>1200 g/cm²
Gelling Temperature
34.5°–37.5° C
Electroendosmosis
0.05–0.13
Sulfate Content
<0.1%
Download product manuals, certificates, and technical resources below.
DTI Agarose Premium - Protocol
Instructions for use and step-by-step protocol.
DTI Agarose Premium - Certificate of Analysis - DT1702.100
Batch-specific quality control results (DT1702.100).
DTI Agarose Premium - Certificate of Analysis - DT1702.500
Batch-specific quality control results (DT1702.500).
Comparative runs of DNA markers on DT1702.500 Agarose premium vs DT1701.500 Agarose Routine.
a) λ Hind III digest DNA Marker run on 0.7% Agarose gel made using TAE buffer
| Lane # | Description | Fragment Size for Reference |
|---|---|---|
| 1, 2 | Cat# 3403, λ Hind III digest DNA Marker | 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min. |
b) 100 bp DNA Marker run on 3% Agarose gel made using TAE buffer
| Lane # | Description | Fragment Size for Reference |
|---|---|---|
| 1, 2 | Cat#DT1801.100, DTI 100bp Ladder (Dye Plus) | 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 base pairs |