DTI FabTaq HS-Glycerol Free (50 U/ul) is a hot start PCR enzyme derived from Thermus aquaticus that includes a neutralizing monoclonal antibody that recognizes Taq DNA polymerase. This antibody binds to Taq DNA polymerase and prevents non-specific amplification due to mispriming and/or formation of primer dimers before thermal cycling. The antibody is denatured during the initial DNA-denaturation step, allowing this product to be used with standard PCR conditions. Since the product is glycerol free, it is suitable for lyophilization processes.
Download product manuals, certificates, and technical resources below.
DTI FabTaq HS-Glycerol Free (50U/ul) - Protocol
Instructions for use and step-by-step protocol.
DTI FabTaq HS-Glycerol Free (50U/ul) - Certificate of Analysis
Batch-specific quality control results.
a) Amplification data: Good performance of this product has been confirmed by PCR using λ DNA as template (amplified fragment size 8 kbp). The protocol used for the assay and the results are as follows:
Figure 1: Amplification data showing product performance with λ DNA as template.
Figure 2: Amplification data for λ DNA (8 kbp amplified fragment size).
b) Hot start function data: Test target sample added to a PCR system containing FabTaq and FabTaq HS respectively. Following an incubation at RT for 30 mins, the products were amplified using a thermal cycler. Non-specific bands (around 280 bp) were observed in the PCR system along with specific target bands (838 bp) using FabTaq, while there were no non-specific bands observed in PCR system using FabTaq HS.
Figure 3: Additional data showcasing product performance.
Figure 4: Hot start function data showing non-specific bands in PCR system with FabTaq and absence of non-specific bands with FabTaq HS.