TaKaRa Bradford Protein Assay Kit

Protein Research

TaKaRa Bradford Protein Assay Kit

The TaKaRa Bradford Protein Assay Kit utilizes the Bradford method with Coomassie dye for rapid and convenient protein quantification within a range of 1 to 1,000 μg/ml.

This assay is based on the principle that Coomassie dye undergoes an absorbance shift from 465 nm to 595 nm upon binding to proteins. Since this shift is proportional to the protein concentration in the solution, protein levels can be determined by measuring absorbance at 595 nm. The resulting protein-dye complex remains stable for 5 to 60 minutes after the reaction begins.

The assay is compatible with samples containing reducing agents; however, the presence of surfactants may lead to inaccurate quantification (refer to Table 1 for the effects of coexisting substances). Additionally, color development may vary depending on the type of protein being measured (see Table 2 for a comparison of color development across different proteins).

Catalog & Pack Sizes

T9310A 500 Rxns

Key Highlights

Storage: Store at 4°C
Content: This kit provides sufficient reagents for up to 500 measurements using a 1 mL reaction or 2,500 measurements with a 200 μL reaction in a microtiter plate. When using the low-concentration protocol, it allows for 1,000 measurements with a 1 mL reaction and up to 5,000 measurements with a 200 μL reaction.

Kit Components:
Bradford Dye Reagent – 250 mL × 2
BSA Standard Solution (2 mg/mL) – 1 mL × 10

Note: The BSA Standard Solution contains 0.9% NaCl and 0.05% NaN₃ for stabilization.
Additional Details:

Additional Required Equipment and Materials:
1. Spectrophotometer with a compatible 1 mL cuvette
2. Microplate reader with a compatible microplate
3. Benchtop centrifuge
4. Microtubes (1.5 mL or 2 mL)
5. A spectrophotometer or a microplate reader

CONTACT US