Takara Taq™ Hot Start Version

PCR Enzyme

Takara Taq™ Hot Start Version

The hot-start versions of Takara Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.

Catalog & Pack Sizes

R007A 250 Units

Details:Hot-Start PCR & Multiplex PCR
Specifications:

1. Takara Taq DNA Polymerase Hot Start Version: Enzyme, buffer, and dNTPs are supplied as separate components.

2. Premix Taq DNA Polymerase Hot Start Version: 2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer.

3. Takara Taq HS Perfect Mix: 2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step.
Bulk, custom, and OEM information: If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information: Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.