The DTI JadeAmp Max HiFid Taq Premix PCR Master Mix offers a powerful and convenient option for high-yield, routine, and specific PCR. This premix formulation includes an optimized buffer, PCR enzyme, dNTP mixture, gel-loading dye (green), and a density reagent in a convenient 2X premix format.
The DTI JadeAmp Max HiFid Taq Premix PCR Master Mix offers a powerful and convenient option for high-yield, routine, and specific PCR. This premix formulation includes an optimized buffer, PCR enzyme, dNTP mixture, gel-loading dye (green), and a density reagent in a convenient 2X premix format. The buffer is optimized for better performance with AT or GC-rich targets, and allows amplification of long products. It is possible to amplify 15 kb genomic DNA fragments with this master mix. Only primers and DNA template need to be added to initiate the reaction. Completed reactions can be analyzed directly via gel electrophoresis. The vivid green dye separates into blue and yellow dye fronts when the PCR product is run on an agarose gel.
Our results showed that the non-hot-start DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix gave minimal background amplification with GC-rich targets. Non-hot-start Competitor(C) Red Mix, on the other hand, resulted in nonspecific amplification and was unable to amplify the target with a high GC content (72.3%). While DTI JadeAmp FabTaq Premix failed to amplify the TGFB-1 gene that is 4 kb long with a 63.1% GC content, DTI JadeAmp Max HiFid Taq Premix was able to generate a clean and distinct band. We were unable to amplify the 4-kb TGFB-1 target using Competitor(C) Red Mix.
We recommend DTI JadeAmp FabTaq Premix (Cat.# DT0201.320). This product is completely premixed, making it easy to prepare PCR reaction mixtures which can be loaded directly on an agarose gel for electrophoresis after the reaction. It includes green tracking dye and a density agent, can amplify targets up to 10 kb (including GC- or AT-rich sequences), and products work directly for restriction enzyme digestion, sequencing, or TA-cloning without purification.
We recommend DTI CobaltAmp HiFid Taq HS Premix for colony PCR. These tolerate substantial bacterial nucleic acid carry-over and include tracking dye and a density agent for direct gel loading.
DTI CobaltAmp HiFid Taq HS Premix is an economical choice for high-throughput projects. It requires only 10 seconds per kilobase extension time, allowing 1 kb colony PCR to complete in under one hour.
DTI FabTaq and DTI FabTaq Hot Start Version enzymes are compatible with inosine-containing primers. They should not be used with enzymes having 3'→5' exonuclease activity; degenerate primer mixtures are recommended instead.
DTI FabTaq and DTI FabTaq Hot Start Version enzymes primarily yield amplification products containing 3'-dA overhangs, which facilitates TA cloning.
Approximately 10⁴ copies of the target DNA sequence are required to detect the amplification product in 25–30 PCR cycles. Specific recommendations: