A loading-dye-added version of JadeAmp FabTaq MAX PCR Master Mix, optimized for standard and high-throughput applications.
DTI JadeAmp FabTaq Premix PCR Master Mix is a loading-dye-added version of JadeAmp FabTaq MAX PCR Master Mix. It contains an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green), and a density reagent in a 2X premix format.
Simply add primers and DNA template. PCR tubes can be loaded directly onto agarose gels or used in downstream applications including restriction enzyme digestion, TA cloning, and direct sequencing.
The product amplifies genomic targets up to approximately 5 kb and works with both GC- and AT-rich targets. The blue dye front appears around 3–5 kb on 1% agarose gel; the yellow dye appears below 50 bp.
Comparison data demonstrates that DTI JadeAmp products outperform competitor red mix on GC-rich targets, achieving minimal background amplification and successful amplification of challenging high-GC targets.
DTI JadeAmp FabTaq Premix and DTI JadeAmp Max HiFid Taq Premix outperformed Competitor (C) Red Mix on GC-rich targets. DTI JadeAmp FabTaq Premix gave minimal background amplification, while the competitor product showed nonspecific amplification on high GC-content targets (72.3%).
DTI JadeAmp Max HiFid Taq Premix successfully amplified a 4 kb TGFB-1 gene (63.1% GC), whereas the competitor product failed.
We recommend DTI JadeAmp FabTaq Premix (Cat.# DT0201.320). This product is completely premixed, making it easy to prepare PCR reaction mixtures which can be loaded directly on an agarose gel for electrophoresis after the reaction. It includes green tracking dye and a density agent, can amplify targets up to 10 kb (including GC- or AT-rich sequences), and products work directly for restriction enzyme digestion, sequencing, or TA-cloning without purification.
We recommend DTI CobaltAmp HiFid Taq HS Premix for colony PCR. These tolerate substantial bacterial nucleic acid carry-over and include tracking dye and a density agent for direct gel loading.
DTI CobaltAmp HiFid Taq HS Premix is an economical choice for high-throughput projects. It requires only 10 seconds per kilobase extension time, allowing 1 kb colony PCR to complete in under one hour.
DTI FabTaq and DTI FabTaq Hot Start Version enzymes are compatible with inosine-containing primers. They should not be used with enzymes having 3'→5' exonuclease activity; degenerate primer mixtures are recommended instead.
DTI FabTaq and DTI FabTaq Hot Start Version enzymes primarily yield amplification products containing 3'-dA overhangs, which facilitates TA cloning.
Approximately 10⁴ copies of the target DNA sequence are required to detect the amplification product in 25–30 PCR cycles. Specific recommendations: